The core’s recharge system is used to cover labor and supply costs for most projects. Other arrangements, such as subcontracts, are available for larger or more complex projects.
Standard pricing is available for certain commonly used protocols. For other work, the core can give you a price estimate prior to starting on your project. Pricing is adjusted periodically to reflect changes in our costs. Users outside of the University of California system will be assessed a 26% surcharge.
RNA quality control
Core users prepare RNA samples and submit these to the core. When sufficient RNA is available for analysis (≥ 10 ng), samples should be analyzed by spectrophotometry (Nanodrop) and by electrophoresis (Agilent Bioanalyzer) prior to preparing sequencing libraries. Agilent Bioanalyzer analysis is offered as a service to users at a cost of $11.27 per sample.
RNA-seq library preparation
The core prepares libraries that are suitable for sequencing on Illumina sequencers. Library preparation includes quality control, normalization, pooling, and sample submission to UCSF sequencing cores. Sequencing costs are not included (see below). For projects with fewer than 4 or more than 48 samples, please contact us to discuss pricing.
|Library type||Method||Cost per sample|
|4-24 samples||25-48 samples|
|PolyA RNA||TruSeq stranded mRNA (>100 ng total RNA)||$393||$373|
|rRNA- and/or globin-depleted RNA||TruSeq stranded Ribo-Zero gold or globin (>100 ng total RNA)||$453||$430|
|Low input total RNA||NuGen Ovation v2
(>50 pg total RNA)
|Small RNA (including miRNA)||TruSeq small RNA (>10 ng total RNA)||$376||$357|
|ChIP-Seq library prep||NexteraXT (>1 ng DNA)||$223||$211|
We submit libraries prepared in our core to the UCSF Center for Advanced Technology (link: http://cat.ucsf.edu) and the UCSF Institute for Human Genetics Genomics Core (link: http://humangenetics.ucsf.edu/genomics-services/). Both Cores have Illumina HiSeq instruments that can run in high output and rapid run modes. Prices are determined by these cores and change periodically to reflect changes in reagent and other costs. We typically aim for 40-50 million reads per sample for standard RNA-Seq projects. Sequencing costs are approximately $150/sample for single-end 50bp sequencing and $400/sample for paired-end 100bp sequencing.
Analysis pricing is charged on a per project basis and is determined by the complexity of the experimental design. Typical costs range from $900-$2500.